Semantic resources project/Antibodies/Revised Methods/Immunodiffusion

Contents

Description

ID: "Immunodiffusion"

  • aka. Agar Gel Immunodiffusion (AGID)

Outline

A qualitative assay used to determine the presence of an antigen by allowing both antigen and antibody to diffuse through a gel.

The presence, thickness, and location of the precipitin line through the gel are the assay's report.

Ontology Terms

MeSH: "Immunodiffusion"

D005779
http://bioportal.bioontology.org/visualize/40401/D005779
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.

HL7v3.0: "Immunodiffusion"

0063
http://bioportal.bioontology.org/visualize/40392/0063

SNOMED: "Immunodiffusion measurement"

32623009
http://bioportal.bioontology.org/visualize/40403/32623009

Usage

Maheswari et al. "Isolation and characterization of an acetyl group-recognizing agglutinin from the serum of the Indian white shrimp Fenneropenaeus indicus". Archives of Biochemistry and Biophysics 402:1,65-76. (June 2002)

"To examine the reactivity of the antiserum against FIA and whole serum of F.indicus by double immunodiffusion (ID) and immunoelectrophoresis (IEP), anti-FIA serum as well as preimmune rabbit serum were fivefold diluted with TBS-II. In ID analyses, 15ll of the sample to be tested was applied to 1% agarose gel prepared in 25 mM borate-buffered saline (pH 8.5) following Ouchterlony. The samples were then allowed to diffuse for 72 h at 24 °C to develop precipitation lines."

Definition

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External Links

  1. http://www.mondofacto.com/facts/dictionary?immunodiffusion
  2. Maheswari et al. "Isolation and characterization of an acetyl group-recognizing agglutinin from the serum of the Indian white shrimp Fenneropenaeus indicus". Archives of Biochemistry and Biophysics 402:1,65-76. (June 2002)