Semantic resources project/Antibodies/Revised Methods/Double Immunodiffusion



DID: Double Immunodiffusion

  • aka. "Ouchterlony Double Immunodiffusion"
  • aka. "passive double immunodiffusion"
  • aka. "agar gel immunodiffusion"

Ontology Terms

OBI: "precipitation"
OBI Browser


An immunodiffusion assay in which an analyte mixture and a detecting antibody (an evaluant) are deposited into two or more wells in a prepared gel. Presence of an antigen which binds the antibody is indicated by the appearance of one or more lines of precipitation (precipitin).


  1. Ouchterlony Double Immuno Diffusion (Wikipedia)
  2. Ouchterlony Test (World of Forensic Science)